AU FOOD ønsker et høj throughput højresolution MS-udstyr optimeret til proteomicskørsler, grundet nye projekter på alternative proteinkilder (eks. single celle protein). AU FOOD har et eksisterende TimTof Pro (2nd generation dual TIMS) udstyr, som ønskes opdateret til en HT (4th generation TIMS XR), der vil give en højere analysehastighed (MS/MS speed ~300 Hz PASEF), øget ionkapacitet, hvilket tillader analyse af øget peptidmængde uden mætning, signifikant forbedret kvantitativ dynamisk range, opnås ved introduktion af Advanced Digitizer Technology (ADT). Det er et ønske at kunne identificerer flere proteiner i komplekse prøver. En opgradering vil resulterer i ~70–76% flere identificerede moder-ioner/precursors. Vi ønsker højere reproducerbarhed i forbindelse med kvantificering af moder-ioner/precursors, og med opdatering til HT forbedres den >2×. HT giver mulighed for mere en halvering af køretiden pr. prøver (30-60 for nuværende udstyr til 5-30 min for HT, hvilket tillader langt mere end en fordobling af kørte prøver per dag (nuværende 10 prøver pr dag til > 50 kørte prøver pr. dag.) HT giver ydermere en forbedret dia-PASEF kørsel i forhold til skala og reproducerbarhed, mulighed for storskala proteomics samt et forbedret injektionssystem med mindre vedligehold og flere kørsler. Opdateringen giver ligeledes et forbedret software, optimeret ved TIMScore™, TIMS DIA NN, ProteoScape™. Det er alene Bruker, der kan tilbyde denne opgradering udført af certificerede teknikere, med efterfølgende garanti på det opgraderede. Givet de høje priser på højresolutions MS-udstyr fra alle leverandører, er vi nået frem til, at en opdatering af eksisterende udstyr er en økonomisk favorabel måde at få udstyr, der lever op til nutidens højeste standard.

AU FOOD aims to acquire high‑throughput, high‑resolution MS equipment optimized for proteomics runs, due to new projects on alternative protein sources (e.g., single‑cell protein). AU FOOD currently operates a TimTof Pro (2nd generation dual TIMS) system, which is intended to be upgraded to an HT (4th generation TIMS XR). This upgrade will deliver higher analysis speed (MS/MS speed ~300 Hz PASEF) and increased ion capacity, enabling the analysis of larger peptide loads without saturation, as well as a significantly improved quantitative dynamic range achieved through the introduction of Advanced Digitizer Technology (ADT). There is a need to identify more proteins in complex samples. The upgrade is expected to result in approximately 70–76% more identified parent ions/precursors. We also seek improved reproducibility in the quantification of parent ions/precursors, which will improve by more than twofold (>2×) with the HT system. The HT system enables more than a halving of the run time per sample (from 30–60 minutes with the current system to 5–30 minutes with HT), allowing for well over a doubling of the number of samples processed per day (from currently 10 samples per day to more than 50 samples per day). Furthermore, HT provides improved dia‑PASEF performance in terms of scalability and reproducibility, enabling large‑scale proteomics, as well as an improved injection system with reduced maintenance and increased runtime. The upgrade also includes improved software optimized with TIMScore™, TIMS DIA NN, and ProteoScape™. Bruker is the only supplier able to provide this upgrade, carried out by certified technicians and followed by a warranty on the upgraded system. Given the high cost of high‑resolution MS equipment from all suppliers, upgrading the existing system has been identified as a cost‑effective way to obtain equipment that meets the highest current standards.