In the past years, techniques have been developed to detect individual molecules using fluorescence – and in particular to add this capability to the simultaneous mechanical manipulation of protein conformation and dynamics, which can be achieved using an optical tweezers instrument. This combination allows for the detection of molecular interactions and dynamics, which is crucial to understanding a host of cellular processes. The aim of the investment is to meet the demands for these state-of-the-art single-molecule fluorescence studies, at the highest light sensitivity, and experimental flexibility. For this purpose, we aim to purchase a confocal fluorescence module that must work in integrated manner with the C-trap optical tweezers instrument by Lumicks, and will be installed in the laboratory at AMOLF. 1 confocal fluorescence module