3x Secventiere Sanger – forward/revers sanger SEQ White prepaid barcodes For a premix of DNA & primer fastest Sanger sequencing service The LightRun tube offers light-speed sequencing of purified plasmids or PCR fragments in 1.5 mL tubes - at a light price. Highlights of the LightRun Tube: • Premixed DNA and primer from you • Reading lengths of up to 1100 Q20 bases Starting material: Purified plasmid DNA or purified PCR fragments with primer premixed in tubes. Data delivery: The following files are delivered by default: • .ab1 files - ABI trace files with all available raw data information • .scf files -Trace files with base calls and quality values • .seq files - Text files with clipped & unclipped FASTA sequences • .phd.1 files - Contains base call and quality information • .pdf quality report - Trace file with quality scores • FASTA file with all sequences Sample requirements: Take 5 µl of the purified template DNA with either of following concentration: • Purified plasmid DNA: 80 - 100 ng/µl • Purified PCR products: 150-300 bp: 2 ng/µl 00-1000 bp: 12 ng/µl >1000 bp: 25 ng/µl Add 5 µl of primer with a concentration of 5 µM (5 pmol/µl) Please send total sample amount of 10 µl in 1.5 ml tubes conform oferta cu Op la 30 de zile Ȋncepând cu data de 01.01.2024, toate persoanele impozabile stabilite în România, indiferent că sunt sau nu înregistrate în scop de TVA, vor avea obligaţia de a raporta facturile prin sistemul RO e-Factura. Dacă nu se poate respecta această obligaţie, vă rugăm să nu daţi curs achiziţiei / notei de comandă!